Device
5UTR RFP

Part:BBa_K3972005:Design

Designed by: Ingrid Kolen, Daniek Hoorn, Werner Doensen   Group: iGEM21_TU-Eindhoven   (2021-09-22)


RFP coding device containing translation enhancing 5UTR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 809
    Illegal AgeI site found at 921
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

First restriction and ligation needed to be performed to insert this composite part in a pSB1C3 plasmid. After ligation the plasmid was transformed into DNA amplifying competent cells.

Source

The pSB1C3 plasmid, containing the RBS-RFP coding device gene (BBa_K801100) was obtained from the 384-well plates from the iGEM distribution kit.

References