Device
5UTR RFP
Part:BBa_K3972005:Design
Designed by: Ingrid Kolen, Daniek Hoorn, Werner Doensen Group: iGEM21_TU-Eindhoven (2021-09-22)
RFP coding device containing translation enhancing 5UTR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 809
Illegal AgeI site found at 921 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
First restriction and ligation needed to be performed to insert this composite part in a pSB1C3 plasmid. After ligation the plasmid was transformed into DNA amplifying competent cells.
Source
The pSB1C3 plasmid, containing the RBS-RFP coding device gene (BBa_K801100) was obtained from the 384-well plates from the iGEM distribution kit.